abc hrp complex (Vector Laboratories)

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Vector Laboratories abc hrp complex
Abc Hrp Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 18030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+abc/VECTASTAIN+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13059316-74-29-31
Average 96 stars, based on 18030 article reviews

abc hrp complex - by Bioz Stars, 2026-09

96/100 stars

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Article Title: NLRP3 Inflammasome Activation and Altered Mitophagy Are Key Pathways in Inclusion Body Myositis
Article Snippet: Stains were visualized using Vectastain ABC (Vector Laboratories, PK‐6100) and Dako DAB chromogen (Agilent, K346811‐2).

Immunodetection:

Article Title: Diagnostic Potential of CD44, CD133, and VDR in Epithelial Ovarian Tumors: Association with Histopathology Parameters.
Article Snippet: Mouse monoclonal antibody (mAb) directed against human CD44 (dilution 1:100 in PBS; 156-3C11, Cell Signaling Technology, Danvers, MA, USA), rabbit mAb directed against human CD133 (dilution 1:50 in PBS; D2VBQ, Cell Signaling Technology, Danvers, MA, USA), and rabbit polyclonal VDR antibody (dilution 1:1000 in PBS; ab3508, Cambridge, MA, USA) were applied overnight at 4 ◦C. .. For the immunodetection of CD133 and VDR, the VECTASTAIN® ABC (Rabbit IgG; Vector Laboratories, Newark, CA, USA) kit with the biotin/avidin system was used according to the manufacturer’s instructions. .. For immunodetection of CD44, goat polyclonal secondary antibody to mouse IgG-HRP (dilution 1:100 in PBS for 60 min; ab6820, Abcam, Cambridge, UK) was applied.

Article Title: Diagnostic Potential of CD44, CD133, and VDR in Epithelial Ovarian Tumors: Association with Histopathology Parameters
Article Snippet: Mouse monoclonal antibody (mAb) directed against human CD44 (dilution 1:100 in PBS; 156-3C11, Cell Signaling Technology, Danvers, MA, USA), rabbit mAb directed against human CD133 (dilution 1:50 in PBS; D2VBQ, Cell Signaling Technology, Danvers, MA, USA), and rabbit polyclonal VDR antibody (dilution 1:1000 in PBS; ab3508, Cambridge, MA, USA) were applied overnight at 4 °C. .. For the immunodetection of CD133 and VDR, the VECTASTAIN ® ABC (Rabbit IgG; Vector Laboratories, Newark, CA, USA) kit with the biotin/avidin system was used according to the manufacturer’s instructions. .. For immunodetection of CD44, goat polyclonal secondary antibody to mouse IgG-HRP (dilution 1:100 in PBS for 60 min; ab6820, Abcam, Cambridge, UK) was applied.

Staining:

Article Title: NAT10-mediated mRNA N 4 -acetylation is essential for the translational regulation during oocyte meiotic maturation in mice.
Article Snippet: .. After several washes in PBS with Tween 20, the sections were stained using Vectastain ABC and DAB peroxidase substrate kits (Vector Laboratories). ..

Article Title: mTOR inhibition in Q175 Huntington’s disease model mice facilitates neuronal autophagy and mutant huntingtin clearance
Article Snippet: Chemical compound, drug , Spurr resin , Electron Microscopy Sciences , Cat# 14300 , . .. Commercial assay kit , Vectastain ABC , Vector Laboratories , Cat# PK-4000; RRID: AB_2336818 , For DAB staining. .. Commercial assay kit , DAB Peroxidase Substrate kit , Vector Laboratories , Cat# SK-4100; RRID: AB_2336382 , For DAB staining.

Article Title: Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth
Article Snippet: Antibody against K i -67 (Cat. #12202, Cell Signaling) was diluted 1:400 with 1% BSA, 0.5% Triton X-100, and 1× PBS and incubated on tissue sections overnight at 4 °C. .. Endogenous peroxidase was blocked with 3% hydrogen peroxide for 30 min at room temperature, followed by biotin-conjugated secondary goat antirabbit antibody diluted 1:250 (Cat. #50-194-1796, Jackson Immuno Research Laboratories) in 0.05% Tween 20 and 1× PBS, VECTASTAIN ABC (Cat. #PK-4000, Vector Laboratories) and AEC (Cat. #SK-4205, Vector Laboratories) staining according to manufacturer instructions. .. Tissues were counterstained with hematoxylin and mounted with aqueous mounting media (Cat. #H-5501, Vector Laboratories).

Article Title: Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth.
Article Snippet: Antibody against Ki-67 (Cat. #12202, Cell Signaling) was diluted 1:400 with 1% BSA, 0.5% Triton X-100, and 1× PBS and incubated on tissue sections overnight at 4 °C. .. Endogenous peroxidase was blocked with 3% hydrogen peroxide for 30 min at room temperature, followed by biotin-conjugated secondary goat antirabbit antibody diluted 1:250 (Cat. #50-194-1796, Jackson Immuno Research Laboratories) in 0.05% Tween 20 and 1× PBS, VECTASTAIN ABC (Cat. #PK-4000, Vector Laboratories) and AEC (Cat. #SK-4205, Vector Laboratories) staining according to manufacturer instructions. .. Tissues were counterstained with hematoxylin and mounted with aqueous mounting media (Cat. #H-5501, Vector Laboratories).

Incubation:

Article Title: Neutrophil ADAM10 promotes migration and inflammation in ARDS by modulating adhesion and chemokine signaling.
Article Snippet: Slides were blocked with Avidin solution (Vector Laboratories, USA) and incubated with rat anti-mouse Ly6G/C antibody (clone RB6-8C5, ab25377, 1:500, abcam) overnight at 4 ◦C. .. Subsequently sections were incubated with biotinylated secondary antibody (BA-4000, Vector Laboratories) for one hour followed by Vectastain ABC (PK-4000, Vector Laboratories) reagent and 3, 3′-diaminobenzidine (DAB) substrate (SK-4100, Vector Laboratories). .. Counterstaining was done using Mayers-Hematoxylin (1.09249, SigmaAldrich).

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